Impact of Genetic Manipulation

Lignification of plant cell walls: impact of genetic manipulation.

A large library of phage-displayed human single-chain Fv antibodies (scFv), containing 6.7 3 109 members, was generated by improving the steps of library construction. Fourteen different protein antigens were used to affinity select antibodies from this library. A panel of specific antibodies was isolated with each antigen, and each panel contained an average of 8.7 different scFv. Measurements...

Endophytic Fungi, Characteristics and Their Potential for Genetic Manipulation

Genetic Manipulation of Bacteria

Genetic manipulation of glycine decarboxylation.

The glycine-serine interconversion, catalysed by glycine decarboxylase and serine hydroxymethyltransferase, is an important reaction of primary metabolism in all organisms including plants, by providing one-carbon units for many biosynthetic reactions. In plants, in addition, it is an integral part of the photorespiratory metabolic pathway and produces large amounts of photorespiratory CO(2) wi...

Genetic Manipulation of Francisella Tularensis

Francisella tularensis is a facultative intracellular pathogen that causes the disease tularemia. F. tularensis subsp. tularensis causes the most severe disease in humans and has been classified as a Category A select agent and potential bioweapon. There is currently no vaccine approved for human use, making genetic manipulation of this organism critical to unraveling the genetic basis of patho...